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Biblioteca(s): |
Embrapa Café. |
Data corrente: |
04/01/2024 |
Data da última atualização: |
04/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MACIEL, A. L. de R.; RODRIGUES, F. A.; PASQUAL, M.; CARVALHO, C. H. S. de. |
Afiliação: |
ANNA LYGIA DE REZENDE MACIEL, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO SUL DE MINAS GERAIS; FILIPE ALMENDAGNA RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; MOACIR PASQUAL, UNIVERSIDADE FEDERAL DE LAVRAS; CARLOS HENRIQUE S DE CARVALHO, CNPCa. |
Título: |
Large-scale, high-efficiency production of coffee somatic embryos. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 16, n. 2, p. 102-107, 2016. |
DOI: |
http://dx.doi.org/10.1590/198470332016v16n2a16 |
Idioma: |
Inglês |
Conteúdo: |
The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos. |
Thesaurus NAL: |
Biotechnology; Coffea arabica var. arabica; Tissue culture; Vegetative propagation. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160431/1/Large-scale-high-efficiency.pdf
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Marc: |
LEADER 01596naa a2200217 a 4500 001 2160431 005 2024-01-04 008 2016 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1590/198470332016v16n2a16$2DOI 100 1 $aMACIEL, A. L. de R. 245 $aLarge-scale, high-efficiency production of coffee somatic embryos.$h[electronic resource] 260 $c2016 520 $aThe study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos. 650 $aBiotechnology 650 $aCoffea arabica var. arabica 650 $aTissue culture 650 $aVegetative propagation 700 1 $aRODRIGUES, F. A. 700 1 $aPASQUAL, M. 700 1 $aCARVALHO, C. H. S. de 773 $tCrop Breeding and Applied Biotechnology$gv. 16, n. 2, p. 102-107, 2016.
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